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complexiolyte 91 (cl-91)  (LOGOPHARM gmbh)

 
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    LOGOPHARM gmbh complexiolyte 91 (cl-91)
    Complexiolyte 91 (Cl 91), supplied by LOGOPHARM gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complexiolyte+91+(cl-91)/complexiolyte+47/bio_rxiv__2024__07__16__603715-212-15-22
    Average 90 stars, based on 1 article reviews
    complexiolyte 91 (cl-91) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Expressing:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Affinity Purification:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Recombinant:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Plasmid Preparation:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Protein Concentration:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Sequencing:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Modification:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Software:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Immunoprecipitation:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Membrane:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Cell Culture:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).

    Incubation:

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: 1038/ s41598- 017- 08144-1 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7 cDNA in pOG1 vector (plasmid) DOI: 10.1073/pnas.0305252101 cRNA synthesis Recombinant DNA reagent Mouse Trpm7- Myc cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI: 10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Mouse Trpm7- HA cDNA in pcDNA3.1/V5- His TA- TOPO vector (plasmid) DOI:10.1073/pnas.0305252101 Expression in mammalian cells Recombinant DNA reagent Human TRPV1- His cDNA in pNKS2 vector (plasmid) This paper See ‘Materials and methods, Antibodies, Molecular biology’ cRNA synthesis Recombinant DNA reagent Mouse Cnnm1- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218318 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm2- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218370 Expression in mammalian cells Recombinant DNA reagent Mouse Cnnm3- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR224758 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Cnnm4-Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR215721 Expression in mammalian cells Recombinant DNA reagent Mouse Arl15- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR218657 Expression in mammalian cells, cRNA synthesis Recombinant DNA reagent Mouse Arl8a- Myc- Flag in pCMV6- Entry (plasmid) OriGene Cat#:MR201740 Expression in mammalian cells, cRNA synthesis Commercial assay or kit Bio- Rad Protein Assay Bio- Rad Cat#:5000006 Protein concentration determination Chemical compound, drug ComplexioLyte CL- 47 Logopharm Cat#:CL- 47–01 Mild detergent buffer Chemical compound, drug ComplexioLyte CL- 91 Logopharm Cat#:CL- 91–01 Detergent buffer with intermediate stringency Chemical compound, drug Trypsin, sequencing grade modified Promega Cat#:V5111 Chemical compound, drug Leupeptin Sigma Cat#:L2884 Continued Continued on next page Kollewe, Chubanov, et al. eLife 2021;10:e68544.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Immunoprecipitation Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: Porcupine controls hippocampal AMPAR levels, composition and synaptic transmission
    Article Snippet: Crude membrane fractions of cultured hippocampal neurons were solubilized in ComplexioLyte buffer CL-91 (Logopharm GmbH) for 30 minutes on ice and incubated with immobilized antibodies for 2 hours at 4°C.

    Article Title: The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics
    Article Snippet: Chemical compound, drug , ComplexioLyte CL-91 , Logopharm , Cat#:CL-91–01 , Detergent buffer with intermediate stringency.

    Article Title: A ternary Neurexin-T178-PTPR complex represents a core-module of neuronal synapse organization
    Article Snippet: Protein complexes in plasma membrane and ER enriched fractions from mouse brains were solubilised with ComplexioLyte 91 (CL-91) or ComplexioLyte 47 (CL-47, Logopharm GmbH) supplemented with 1 mM CaCl 2 (and protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF)).



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